FastQCFastQC Report
Thu 2 Jun 2016
DRR028598_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028598_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572581
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.045.0074771
TCAACGC13600.040.785794
ATCAACG14100.039.857113
GTATCAA17350.036.1770481
GTACATG32200.033.545941
CAACGCA16800.033.450425
TCTCGGG14050.033.18109145
AACGCAG18400.030.5406997
CGCAGAG19550.028.3681328
TACATGG33200.028.3586672
ACGCAGA20250.028.1082298
TATCAAC22200.027.615982
ACATGGG33850.027.3828873
CTTAGGG17050.024.81883145
TAGCTCG1550.001740420523.5428165
CGGTGGG15000.022.472958145
CCTAACG1650.00276960521.734001145
TCGCCGG8150.020.240622145
GAGTACA28550.019.6842461
CCGGTCG3351.235845E-619.6047639
Produced by FastQC (version 0.11.5)