FastQCFastQC Report
Thu 2 Jun 2016
DRR028598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572581
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.074.282521
GTATCAA37850.055.897961
TCAACGC39200.052.4669084
ATCAACG39400.052.200583
CAACGCA42150.048.7948465
AACGCAG44400.046.3221326
TAGACCG657.378327E-444.565614
TATCAAC47550.043.4057732
CGCAGAG49050.041.4878128
ACGCAGA49050.041.3401687
GCAGAGT58650.033.091779
GTACATG29550.028.7870561
GTATACG2155.9784725E-727.0533081
GTGGTAT11100.026.8552821
TGGTATC10900.025.247032
GTTACGA1751.2322277E-424.927691
TCTGTCG5050.024.378718
ACATGGG33000.023.9202543
TACATGG33600.023.7086372
CAGAGTA57850.022.2828039
Produced by FastQC (version 0.11.5)