FastQCFastQC Report
Thu 2 Jun 2016
DRR028597_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028597_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879728
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22550.048.866364
ATCAACG23000.047.592993
GGTATCA15900.046.8147431
GTATCAA26300.044.3961
GTACATG47800.039.388681
CAACGCA30000.037.2177165
ACGCAGA29750.035.8124247
TATCAAC31600.035.795152
AACGCAG31250.035.4954876
TACATGG50350.035.219762
CGCAGAG30400.034.0837448
ACATGGG50150.032.449913
TAGACCG1153.0440465E-431.7286625
CTTAGGG21950.028.429548145
GTGGTAT11000.025.2098641
TCTCGGG20700.024.94867145
TGACGCG3501.4915713E-1024.592258145
CATGGGG38000.024.1972794
GTCGTTC1550.001742591823.538078
GAGTACA38500.023.5039331
Produced by FastQC (version 0.11.5)