FastQCFastQC Report
Thu 2 Jun 2016
DRR028597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879728
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.072.5826951
GTATCAA40100.054.783151
TCAACGC44850.047.7973064
ATCAACG45050.047.585113
CAACGCA48050.044.764865
AACGCAG51200.042.010776
TATCAAC52550.041.344972
ACGCAGA55550.038.069137
CGCAGAG56400.037.2385758
GTACATG46550.032.190821
GTGGTAT12250.030.8781811
GCAGAGT68550.030.1100549
TACATGG49050.028.7918622
ACATGGG49850.027.1675593
GTACGGA2051.3017809E-524.729676
TGGTATC13800.024.140872
CAGAGTA62300.022.4358969
CGTGTCG2003.1269368E-421.726782145
GAGTACA38300.021.6516281
TCTGTCG6500.021.1696858
Produced by FastQC (version 0.11.5)