FastQCFastQC Report
Thu 2 Jun 2016
DRR028595_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028595_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867898
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.051.1877371
GTATCAA26800.050.6813281
ATCAACG26000.049.9938353
TCAACGC26200.049.3334854
CAACGCA31900.040.747335
GTACATG52550.039.743231
TATCAAC33850.039.2629132
TACATGG52600.038.0394862
AACGCAG34100.037.904326
ACGCAGA33900.037.264267
CGCAGAG36050.034.6329548
ACATGGG58350.033.0394553
GCAGAGT41850.026.8665649
CATGGGG42500.026.2888954
TCTCGGG20900.024.68734145
CTTAGGG21900.024.54173145
TACGACG1500.001433269924.341575
GTATACG2751.8379251E-723.8989941
GAGTACA41350.023.6645971
AGTACAT40900.022.6751522
Produced by FastQC (version 0.11.5)