FastQCFastQC Report
Thu 2 Jun 2016
DRR028595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867898
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.077.326471
GTATCAA42450.057.033891
TCAACGC47450.050.368064
ATCAACG48150.049.635813
CAACGCA51050.046.674285
TATCAAC54650.043.599672
AACGCAG55100.043.2435956
ACGCAGA61900.038.1420867
GTACATG52850.037.969121
CGCAGAG62900.037.190278
TACATGG54750.035.0541342
ACATGGG56500.031.6610973
GCAGAGT69850.031.623579
GTGGTAT12000.027.870391
CATGGGG43150.024.0011864
CATGGGA24850.022.732424
CAGAGTA67350.022.47439
GAGTACA43550.021.36921
TGGTATC14450.020.0479342
AGTACAT44000.018.928782
Produced by FastQC (version 0.11.5)