FastQCFastQC Report
Thu 2 Jun 2016
DRR028594_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028594_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612913
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14900.054.8302154
ATCAACG15800.051.706983
GGTATCA11150.050.3737261
GTATCAA20050.044.020931
CAACGCA18900.043.224585
AACGCAG19650.041.9459886
ACGCAGA22000.037.4630367
CGCAGAG21950.037.2149128
TATCAAC22200.037.129042
GTACATG33250.035.9782831
TACATGG31700.032.675132
TCTCGGG13750.029.761213145
TCGCCGG8700.028.882145
CTTAGGG18650.028.485992145
ACATGGG35650.028.4409123
ATATCGC1100.00939120726.5241746
GCAGAGT30300.025.9948489
AACCGCG1450.001184538125.150657
GTCGTAC1751.20519195E-425.0092951
GTACGGA1801.4591575E-424.3145921
Produced by FastQC (version 0.11.5)