FastQCFastQC Report
Thu 2 Jun 2016
DRR028594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612913
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.11941127636766521No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17040.10564735977699977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.075.454571
GTATCAA38950.055.1068761
ATCAACG42050.050.1242833
TCAACGC42650.049.4191364
CAACGCA44800.047.0474555
AACGCAG46100.045.8778536
TATCAAC47850.044.3513532
CGCAGAG53000.039.6317568
ACGCAGA54400.038.744977
GTGGTAT12600.034.0700451
GCAGAGT62700.033.269499
GTACATG33050.032.8024371
TATATCG1352.1571203E-532.191315
TACATGG34000.030.2503662
GTCGTAC1306.350472E-427.9845241
TGGTATC13550.027.7961882
ACATGGG33800.027.4292853
TAGCTCG1751.2644046E-424.8332989
CATGGGG24900.022.3981724
CAGAGTA63700.020.9218259
Produced by FastQC (version 0.11.5)