FastQCFastQC Report
Thu 2 Jun 2016
DRR028593_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028593_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236046
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC18550.057.2538145
TCAACGC19000.050.3052254
ATCAACG19800.048.6411823
GTATCAA24200.044.319751
CAACGCA22650.042.1976785
GGTATCA15150.041.8988381
AACGCAG24150.039.5758066
ACGCAGA25050.036.696837
CGCAGAG24550.036.2522168
CGACGAA6000.035.880337145
TATCAAC28050.035.635522
GTACATG45800.034.8878331
TACATGG47850.030.496042
ACATGGG50000.026.9958553
GAGTACA34550.021.540091
GCAGAGT39200.021.2150979
TCTCGGG33250.020.718872145
CATGGGG38850.019.7193984
CATGGGA23850.019.272924
TACACGC3201.8273953E-518.2400285
Produced by FastQC (version 0.11.5)