FastQCFastQC Report
Thu 2 Jun 2016
DRR028593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236046
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.063.625811
GTATCAA44850.056.2431261
ATCAACG44400.055.2960743
TCAACGC45100.054.277244
CAACGCA46950.052.6012845
AACGCAG49200.050.1957366
TATCAAC54450.045.621952
ACGCAGA54850.044.497017
CGCAGAG55750.043.388958
GCAGAGT68250.034.699469
GTACATG45450.026.550741
TACATGG49450.022.8473532
GTGGTAT18900.022.6930371
ACATGGG46500.021.9605773
TCTGTCG6800.021.3009368
GAGTACA39300.021.0869981
CAGAGTA66650.021.0804149
CTGTCGC7400.020.5525239
TATACGT3051.2878154E-518.9962444
TATACTG17700.018.8218425
Produced by FastQC (version 0.11.5)