FastQCFastQC Report
Thu 2 Jun 2016
DRR028592_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028592_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930556
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17200.049.1902584
ATCAACG17400.048.6248473
CGATACC16350.043.47272145
CAACGCA20500.041.983415
GTATCAA23850.039.756171
GGTATCA13850.038.9700971
AACGCAG21850.038.721856
ACGCAGA24100.034.802267
CGCAGAG24450.034.003098
TATCAAC27000.032.6867072
GTACATG38100.031.9698811
TACATGG40550.027.6999762
TATACCG3005.475158E-1026.7436686
TACCGCC3851.4551915E-1124.624938
GTACGGA3001.6034392E-824.3124266
ATACCGC3301.6570993E-924.3111447
CGACGAA5050.024.168901145
ACATGGG42600.023.9699973
TTTCGCG1600.002104026722.7875029
AGTACGG3604.5292836E-922.2863885
Produced by FastQC (version 0.11.5)