FastQCFastQC Report
Thu 2 Jun 2016
DRR028592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930556
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.067.045421
GTATCAA38100.052.1073271
TCAACGC37050.051.997814
ATCAACG37450.051.442423
CAACGCA39250.049.0832825
AACGCAG42100.046.2766346
TATCAAC46500.041.586262
ACGCAGA47100.040.4414257
CGCAGAG47050.040.1765378
ACCGCGT1051.8655886E-434.488348
GCAGAGT58700.031.832689
GTACATG38350.031.4777621
TACATGG41950.026.9329682
GTGGTAT14050.026.3970241
GAGTACA34350.025.404791
ACATGGG44550.024.7108363
GTACGGA1801.5316434E-424.1418386
TGGTATC14100.023.6281812
TATACGT2003.1261312E-421.7276544
AGTACAT37600.021.5735572
Produced by FastQC (version 0.11.5)