FastQCFastQC Report
Thu 2 Jun 2016
DRR028591_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028591_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718226
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC15450.055.2526145
ATCAACG16500.051.309033
TCAACGC16850.051.109534
GGTATCA13400.044.1163941
CAACGCA19950.043.166425
ACGCAGA20550.041.1909337
GTATCAA23000.040.2991261
CGCAGAG20850.040.2435078
AACGCAG21550.039.281856
TATCAAC24500.035.4487272
GTACATG35000.034.4061161
TACATGG36850.030.896332
GTACGGA2751.9826984E-1029.1904756
ACATGGG40600.026.4247633
CTACCGT2101.478977E-524.3253966
GCAGAGT31250.024.048689
ACCGTAC1550.001743196723.5365248
TAGATCG1902.0980462E-423.0464765
AGTACGG3803.3469405E-1023.0464765
CGACGAA5000.022.955368145
Produced by FastQC (version 0.11.5)