FastQCFastQC Report
Thu 2 Jun 2016
DRR028591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718226
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.076.368861
GTATCAA36750.056.9709241
TCAACGC38900.052.4999544
ATCAACG39000.052.3653343
CAACGCA41600.049.266595
AACGCAG44350.045.8851476
TATCAAC45500.045.2029082
CGCAGAG49650.039.9660038
ACGCAGA50000.039.831087
GCAGAGT59950.032.4954459
GTACATG37050.031.3942381
GTCGTAC1000.005995521329.078911
TACATGG37950.029.0062262
TCACCGG4250.027.264055145
ACATGGG41200.026.0150053
GTGGTAT11000.023.7918381
TATATCG1650.002618311221.94555
CAGAGTA57600.021.625469
CATGGGA18350.020.9169924
TACAGCG1750.003683226620.6914715
Produced by FastQC (version 0.11.5)