FastQCFastQC Report
Thu 2 Jun 2016
DRR028590_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028590_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230708
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33300.055.4487151
ATCAACG53250.054.5479773
TCAACGC53900.053.8901674
GTATCAA59900.050.5634231
CGATACC40000.048.77866145
TATCAAC68850.043.248542
GTACATG108750.043.0173841
CAACGCA69200.042.080615
TACATGG106000.041.9301722
ACGCAGA69250.040.991177
AACGCAG72500.040.1639486
CGCAGAG71050.038.8192528
ACATGGG117000.036.179063
GCAGAGT79900.032.05189
CATGGGG71900.031.9739954
CATGGGA51150.029.2498364
CGACGAA11000.028.041206145
AGTACAT79550.026.1468812
GAGTACA84200.025.7430251
GTACGGA5400.025.6780136
Produced by FastQC (version 0.11.5)