FastQCFastQC Report
Thu 2 Jun 2016
DRR028590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230708
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35050.10849015138477386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32450.10044237981272217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45000.079.3485951
GTATCAA85550.057.294131
ATCAACG95150.050.4665533
TCAACGC95900.050.1473964
CAACGCA104050.046.2194635
TATCAAC109550.044.0973362
AACGCAG110400.043.4954036
ACGCAGA119250.039.8422977
CGCAGAG122050.038.6908878
GTACATG103250.037.7524341
TACATGG103050.036.055232
GTGGTAT21150.032.6650621
GCAGAGT136600.032.5549169
ACATGGG110550.032.298853
CATGGGG73100.027.0485614
CATGGGA48450.024.9644264
TGGTATC26900.024.5012362
AGTACAT83150.024.3019352
GAGTACA88000.024.2133661
CAGAGTA137050.021.8786339
Produced by FastQC (version 0.11.5)