FastQCFastQC Report
Thu 2 Jun 2016
DRR028589_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028589_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794951
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16850.052.825874
ATCAACG16950.052.514213
CGATACC14800.051.393375145
CAACGCA18950.046.58685
GTATCAA21950.042.878731
GGTATCA13350.042.0819851
AACGCAG21000.041.6916126
TATCAAC25350.036.5521132
CGCAGAG22400.036.477068
ACGCAGA23450.036.4023787
GTACATG38350.032.722751
CGACGAA4050.030.12013145
TACATGG40450.029.5809462
ACATGGG40900.027.653
CCGGTCG3108.0035534E-1025.8854799
GAGTACA30400.022.80011
GCAGAGT32450.021.8063139
CTATACG3055.0292147E-721.5292764
TCTCGGG26000.020.699074145
TAGCGCA1800.004156766520.2638859
Produced by FastQC (version 0.11.5)