FastQCFastQC Report
Thu 2 Jun 2016
DRR028589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794951
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.073.769311
GTATCAA41100.057.523211
TCAACGC43050.053.336024
ATCAACG44050.052.289653
CAACGCA45200.050.7990235
AACGCAG48600.047.2451826
TATCAAC49750.046.8810462
CGCAGAG55050.041.0517588
ACGCAGA56300.040.3976177
GTACATG39450.033.1915131
GCAGAGT66850.032.9387179
TACATGG42250.029.6588232
GTGGTAT15400.027.8697361
TGGTATC12000.027.7658582
ACATGGG40600.026.7608383
GTATACG2003.034439E-421.8234211
CAGAGTA64250.021.7579869
GTATAAC7900.021.1788441
GAGTACA30900.020.2461071
CATGGGG29300.020.0240484
Produced by FastQC (version 0.11.5)