FastQCFastQC Report
Thu 2 Jun 2016
DRR028588_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028588_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922943
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17200.053.442574
ATCAACG17850.051.4964833
CAACGCA20150.045.6184735
GTATCAA22400.043.641741
CGATACC14200.042.96056145
AACGCAG22350.041.454486
GGTATCA14400.037.996541
CGCAGAG24500.036.323958
ACGCAGA24850.036.10787
TATCAAC25900.036.0541652
GTACATG37600.031.2380051
TACATGG38600.028.5387462
ACATGGG41750.024.288663
GCAGAGT35350.022.4918379
ACCGCGT2104.1412454E-420.8416128
CGACGAA3901.3720637E-820.242651145
TACGAGT4102.0198058E-819.5728514
GAGTACA31700.018.8712141
CATGGGG29300.018.6740674
TCTCGGG24250.018.349289145
Produced by FastQC (version 0.11.5)