FastQCFastQC Report
Thu 2 Jun 2016
DRR028588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922943
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19270.10021097869255616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24550.070.20161
GTATCAA42600.056.673321
TCAACGC47150.049.9265944
ATCAACG47050.049.7248083
CAACGCA48950.047.942715
AACGCAG50950.046.0607576
TATCAAC55750.042.7446022
CGCAGAG59600.038.6465848
ACGCAGA60000.038.5096637
GTACATG36550.031.4354631
GCAGAGT72950.030.879159
ATACCGC2003.283858E-728.9727887
TACATGG39650.025.2096122
GAACCGT2101.5673922E-524.1439886
TACGCGC1550.001819000923.365156
CAGAGTA70150.023.1286669
ACATGGG40850.022.695943
TATAACG1650.002615944721.949082
TATACCG2704.4847257E-621.4613236
TACCGCC3406.503433E-821.303528
Produced by FastQC (version 0.11.5)