FastQCFastQC Report
Thu 2 Jun 2016
DRR028587_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028587_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735358
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.053.425111
TCAACGC18350.047.7237744
ATCAACG18550.047.209233
CGATACC14450.046.16826145
GTATCAA23100.040.43781
CAACGCA21750.040.262325
AACGCAG23700.036.94966
GTACATG35850.036.4379121
ACGCAGA25100.034.5979277
CGCAGAG25650.033.2851038
TATCAAC26900.032.8263552
TACATGG38400.031.92772
CGACGAA4950.031.882025145
ACATGGG41950.027.3122373
GCAGAGT31850.024.7430349
GTACGGA1700.002978574521.463376
TACACGC2404.1507163E-521.2845085
CCGCGCG2454.868795E-520.8482999
CATGGGG31900.020.589294
ACCCGAC1850.00486103519.7230977
Produced by FastQC (version 0.11.5)