FastQCFastQC Report
Thu 2 Jun 2016
DRR028587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735358
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.1017657451661271No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22250.073.216721
GTATCAA38600.055.7694931
TCAACGC41450.051.2041444
ATCAACG42000.050.3611373
CAACGCA44100.048.127255
AACGCAG45950.046.3472376
TATCAAC48150.043.9287222
ACGCAGA52400.039.9510847
CGCAGAG53300.039.004688
GTACATG35300.033.3758661
GCAGAGT64000.032.2572179
ACCGGTC1204.079905E-430.1821928
TACATGG39200.029.1966512
TGGTATC13750.027.921272
GTGGTAT14150.027.7542571
ACATGGG40300.027.3212493
GTATACG1606.687481E-527.2724111
GAGTACA30550.022.8534771
TATACGG1600.002186764722.6366442
TATAACG2053.6894507E-421.2011492
Produced by FastQC (version 0.11.5)