FastQCFastQC Report
Thu 2 Jun 2016
DRR028586_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028586_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755407
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.048.3601571
TCAACGC18500.045.2475364
CGATACC16750.044.720848145
ATCAACG20050.042.1126373
GTATCAA22300.039.495661
CAACGCA22950.037.425555
GTACATG37200.037.3731161
AACGCAG25350.033.595166
ACGCAGA25300.033.0842367
CGCAGAG25950.032.5350848
TATCAAC27550.031.4408442
TACATGG41650.031.282892
ACATGGG44100.029.0497823
GCAGAGT31000.023.7132459
GTATATA13900.023.564951
GTACGGA2303.0484964E-522.1533326
TCTAGCG1650.002542114822.0574282
CGACGAA5250.021.950893145
GAGTACA32400.020.6686291
GTATAGG9350.020.2409361
Produced by FastQC (version 0.11.5)