FastQCFastQC Report
Thu 2 Jun 2016
DRR028586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755407
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.068.929971
GTATCAA38100.053.2670251
ATCAACG40150.049.425093
TCAACGC40650.048.995324
CAACGCA42100.046.963775
AACGCAG45400.043.3905876
TATCAAC49450.040.1297762
ACGCAGA51350.038.2218137
CGCAGAG51350.037.9397358
GTGGTAT12250.034.4406361
GTACATG36700.031.514491
GCAGAGT61250.030.861499
TCGGATA1050.00774957627.5900922
TACATGG41000.025.966652
TGGTATC14750.025.0415152
ACATGGG43150.023.8336183
GTACGGA2451.9309882E-623.6486516
CAGAGTA59100.020.3424429
TTTCGCG2908.330489E-619.9790339
GCCGTGC5505.456968E-1219.7519978
Produced by FastQC (version 0.11.5)