FastQCFastQC Report
Thu 2 Jun 2016
DRR028585_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028585_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228316
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG28500.054.7816163
TCAACGC28650.054.2389144
CGATACC20350.050.074314145
GTATCAA33100.049.152181
GGTATCA17850.047.8204421
CAACGCA34450.045.3190235
AACGCAG37300.041.660726
TATCAAC39200.040.945162
ACGCAGA37700.040.4437147
CGCAGAG38550.038.6057438
GTACATG58900.038.5221941
TACATGG60050.034.8686262
ACATGGG63550.031.3410113
GCAGAGT45400.029.0829939
GAGTACA45150.024.5613231
CATGGGG45050.023.967514
AGTACAT46600.022.5445982
ATATACG1952.5072636E-422.4482563
TTTCGCG3604.5220077E-922.289829
CATGGGA29400.021.836924
Produced by FastQC (version 0.11.5)