FastQCFastQC Report
Thu 2 Jun 2016
DRR028585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228316
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.073.373031
GTATCAA50700.059.7894481
TCAACGC55350.052.9995084
ATCAACG56100.052.420073
CAACGCA58250.050.3609055
AACGCAG62100.047.3553356
TATCAAC66850.044.207222
ACGCAGA69100.041.824367
CGCAGAG69900.041.0348138
GTACATG58850.038.5393681
GCAGAGT80150.034.4314969
TACATGG60400.034.177662
ACATGGG62200.031.0924783
TAGATCG1902.0955304E-730.4979655
ATACCGC2202.2873792E-829.6315447
GAGTACA48850.025.1488761
CATGGGG41400.025.019014
GTGGTAT14900.024.881721
TACGCGC2351.3424869E-624.6579276
TATACCG2701.6656486E-724.144226
Produced by FastQC (version 0.11.5)