FastQCFastQC Report
Thu 2 Jun 2016
DRR028584_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028584_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705121
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.054.541321
TCAACGC22050.049.973314
ATCAACG22200.049.6356473
CGATACC17150.047.690887145
GTATCAA25300.046.7266041
CAACGCA25850.042.6258625
AACGCAG27200.040.5102426
CGACGAA4600.037.432438145
TATCAAC30200.036.9704062
GTACATG44450.036.117751
ACGCAGA29450.035.927467
CGCAGAG30150.033.8791668
TACATGG44900.033.6429252
ACATGGG47550.029.7728883
GCAGAGT36600.024.91779
GAGTACA34500.024.1132351
CATGGGG31300.022.6150284
ACTACGC2353.533942E-521.7331248
AGTACAT35050.021.6528472
TATATCG1750.003524659720.8491695
Produced by FastQC (version 0.11.5)