FastQCFastQC Report
Thu 2 Jun 2016
DRR028584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705121
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.10362900931957322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21800.071.72231
GTATCAA40250.056.5523531
TCAACGC43950.050.925634
ATCAACG44000.050.867763
CAACGCA47150.047.315765
AACGCAG49050.045.6306086
TATCAAC51250.043.95452
ACGCAGA54950.040.072157
CGCAGAG56200.039.180878
GTACATG45100.037.732151
TACATGG45750.035.939462
GTGGTAT12100.034.2579771
GCAGAGT64300.032.5554479
ACATGGG49250.032.5029533
TGGTATC12650.028.0570722
GTACGGA3005.9299055E-1026.5587846
CATGGGA21550.025.5448364
AGTACGG3652.2191671E-1023.8136065
CATGGGG35250.023.0141894
GAGTACA36850.022.6951261
Produced by FastQC (version 0.11.5)