FastQCFastQC Report
Thu 2 Jun 2016
DRR028583_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028583_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755775
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC21150.054.1584174
ATCAACG21750.052.664393
GGTATCA14050.052.447131
GTATCAA24750.048.3443031
CAACGCA25100.045.6354755
CGATACC16050.045.162205145
AACGCAG26700.042.8995256
TATCAAC28850.040.7152142
ACGCAGA28850.037.9301347
CGCAGAG28950.036.7868778
GTACATG45400.035.0329971
TACATGG46100.034.0262572
CGACGAA4850.032.554405145
ACATGGG51450.029.2118113
GTACGGA3800.026.8787166
GAGTACA37600.026.777371
GCAGAGT35650.026.5978959
AGTACAT36250.025.7619462
GTCTTAC8500.024.8917681
CATGGGG36500.024.1862854
Produced by FastQC (version 0.11.5)