FastQCFastQC Report
Thu 2 Jun 2016
DRR028583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755775
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.070.918141
GTATCAA41000.057.2727741
TCAACGC42700.053.7666444
ATCAACG43050.053.3295143
CAACGCA46750.049.108785
AACGCAG48550.047.2880676
TATCAAC49600.046.433022
ACGCAGA56350.040.4853747
CGCAGAG57200.039.6305248
GTACATG42750.038.0926741
TACATGG43800.034.889112
TAGACCG850.00275671634.0818065
GCAGAGT65950.033.7135969
ACATGGG45200.032.847093
GTGGTAT14350.031.4101031
TGGTATC14000.027.934912
CATGGGG32200.026.765334
GAGTACA34750.024.2679311
GAGCGCG2151.882023E-523.5798519
TAGATCG1550.001820126923.3625285
Produced by FastQC (version 0.11.5)