FastQCFastQC Report
Thu 2 Jun 2016
DRR028582_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028582_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066834
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.048.791421
TCAACGC21700.045.0569464
ATCAACG21700.044.72073
GTATCAA26050.041.1743051
GTACATG46950.037.6094061
CAACGCA28500.034.0504955
TATCAAC29700.033.6574172
TACATGG49400.032.346992
AACGCAG30200.032.1337476
GTACGGA3050.031.0999856
ACGCAGA31350.030.2552747
CGCAGAG32650.029.272658
GAGTACA37200.027.8523641
ACATGGG54250.027.7066573
TAGTACG3709.094947E-1225.6364734
AGTACGG4353.6379788E-1223.48315
CATGGGG38250.022.3188134
AGTACAT42300.022.2518442
ACGGATG4357.6397555E-1121.803598
CATGGGA23050.021.2090174
Produced by FastQC (version 0.11.5)