FastQCFastQC Report
Thu 2 Jun 2016
DRR028582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066834
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23250.074.740521
GTATCAA43600.056.6987651
ATCAACG44600.053.916443
TCAACGC44750.053.5738564
CAACGCA49050.048.8772745
TATCAAC50650.047.619272
AACGCAG52600.045.4408236
ACGCAGA58450.040.7689367
CGCAGAG58400.040.555798
GTACATG49600.034.5948521
GCAGAGT68600.033.6809659
TACATGG52250.031.6057822
ACATGGG54000.029.5084843
GAGTACA39400.023.9898931
CATGGGG39750.022.7767014
GTATTAG15500.022.046891
AGTACAT43150.021.3177262
GTACGAC1700.003111300821.3029173
TAGCGTT2755.258971E-621.070525
GTGGTAT14300.020.3378541
Produced by FastQC (version 0.11.5)