FastQCFastQC Report
Thu 2 Jun 2016
DRR028581_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028581_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235691
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.055.0952221
TCAACGC28250.049.331384
ATCAACG29250.047.6448363
GTATCAA34450.043.2065161
GTACATG58300.042.176421
TACATGG61100.038.5717772
CAACGCA38650.036.0572175
TATCAAC39950.035.4318052
ACGCAGA39000.034.983697
CGCAGAG38600.034.5869948
ACATGGG65800.034.153343
AACGCAG41000.033.8117946
GTACGGA3251.8189894E-1229.1849166
GCAGAGT47650.024.1898619
GAGTACA45800.024.05581
CATGGGG39500.024.0134494
CATGGGA31350.023.5067274
AGTACAT47500.022.2732072
AGTACGG4657.2759576E-1221.967645
TACACGC5301.8189894E-1220.6501735
Produced by FastQC (version 0.11.5)