FastQCFastQC Report
Thu 2 Jun 2016
DRR028581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235691
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.076.294541
GTATCAA52250.054.7017021
ATCAACG54300.051.089533
TCAACGC54300.050.8227464
CAACGCA60400.045.9298255
TATCAAC61450.045.2628942
AACGCAG64800.042.9229136
ACGCAGA70650.038.7536477
CGCAGAG70200.038.6925288
GTACATG55150.036.923861
TACATGG58750.034.2744032
GCAGAGT79600.032.667389
ACATGGG62800.030.5646323
GTGGTAT13850.028.8806841
TGGTATC14700.026.6078262
CATGGGA30450.022.3600884
CATGGGG37900.021.7870584
GAGTACA49900.021.278791
CAGAGTA80750.020.5412049
GCCGTGC7550.020.1467658
Produced by FastQC (version 0.11.5)