FastQCFastQC Report
Thu 2 Jun 2016
DRR028580_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028580_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571381
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13600.048.8308454
ATCAACG13650.048.6519783
GGTATCA11650.042.596581
GTATCAA16950.042.1937521
CAACGCA15900.041.7645575
AACGCAG17150.039.1459966
ACGCAGA17800.036.894197
GTACATG26350.036.558221
TATCAAC20250.034.2365762
CGCAGAG18900.033.973668
TACATGG29000.032.2109642
GTCCGCG900.003821652831.883354145
TAGATCG1504.2092546E-529.1892975
ACATGGG31350.027.4685823
CGACGAA1959.507556E-625.75194145
TCCGACA1650.002504107822.1145342
TATATCG1650.002505049822.1131025
GCAGAGT27900.021.1816649
GTACGGA2154.8407656E-420.3646246
CCGGTCG2907.816043E-620.126649
Produced by FastQC (version 0.11.5)