FastQCFastQC Report
Thu 2 Jun 2016
DRR028580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571381
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.11741264531008075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17870.11372162448190477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20600.075.167821
GTATCAA35200.057.827571
TCAACGC39450.050.484464
ATCAACG40650.048.994143
CAACGCA41550.047.75865
AACGCAG42650.046.5268366
TATCAAC45800.044.117482
ACGCAGA49700.039.4897967
CGCAGAG49950.039.147168
GCAGAGT61050.031.0804719
GTACATG25550.028.7375721
GTGGTAT12250.024.3314151
GTACGGA2101.5687834E-524.140756
CAGAGTA58400.023.8100539
TACATGG28450.023.1649362
ACACCGT1952.6347238E-422.2837686
ACATGGG28550.021.8154563
TGGTATC12400.021.6098652
CCGGTCG2908.331306E-619.9785529
CCGGATA2359.2698424E-418.4907864
Produced by FastQC (version 0.11.5)