FastQCFastQC Report
Thu 2 Jun 2016
DRR028578_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028578_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074303
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG26700.050.8311463
TCAACGC26900.050.4532174
GTATCAA29700.046.679411
GGTATCA17350.045.420561
CAACGCA33050.040.843025
GTACATG53850.040.1082951
TATCAAC36300.037.9912342
AACGCAG35900.037.8038526
ACGCAGA36500.036.3827977
TACATGG57450.036.070862
CGCAGAG37800.033.7794958
ACATGGG63900.031.4021973
CATGGGG40500.027.5654184
GTACGGA2954.4929038E-1027.2074726
TATACCG2456.0575985E-826.8036545
CATGGGA23150.025.8458634
GCAGAGT46450.025.2892769
ATACCGT3251.380613E-924.6960146
GAGTACA45050.022.9996781
GTGGTAT12500.022.1820561
Produced by FastQC (version 0.11.5)