FastQCFastQC Report
Thu 2 Jun 2016
DRR028578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074303
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28600.078.5659941
GTATCAA51650.055.4712641
ATCAACG56900.049.3938563
TCAACGC57350.048.8799824
CAACGCA62050.045.2942855
TATCAAC64500.043.9107132
AACGCAG64950.043.2719086
ACGCAGA71850.039.2171757
GTACATG50100.038.899071
CGCAGAG73600.037.595778
TACATGG54350.033.9855352
ACATGGG56300.031.521813
GCAGAGT83500.031.316579
TAGATCG1555.5365323E-528.0396775
TAGTACG4450.027.6721154
GTGGTAT15000.027.6328531
CATGGGG39050.025.9693134
GTACGGA3001.7254933E-824.1452776
GAGTACA42500.023.7830771
ACTATCG1550.001818484223.3663988
Produced by FastQC (version 0.11.5)