FastQCFastQC Report
Thu 2 Jun 2016
DRR028577_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028577_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875118
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.053.8042561
TCAACGC24850.047.2689324
ATCAACG25300.046.7165573
GTATCAA28900.042.159451
CAACGCA29100.040.3653955
GTACATG45800.040.302441
AACGCAG30600.038.1472366
TACATGG48250.037.197532
ACGCAGA31950.035.6200687
TATCAAC35000.034.1863022
CGCAGAG33400.033.6359338
ACATGGG52400.031.8845673
GTACGGA3250.031.4274566
AGTACGG4000.029.1834265
GTACCGA1606.545384E-527.3587236
CATGGGG32900.025.2804784
GCAGAGT40100.024.012389
CATGGGA24300.022.8183174
CGGATGC4800.022.7958459
AGTACAT35250.022.560242
Produced by FastQC (version 0.11.5)