FastQCFastQC Report
Thu 2 Jun 2016
DRR028577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875118
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.1075132338338174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18880.10068699676500359No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.077.778851
GTATCAA46350.054.5747071
ATCAACG50600.049.6611253
TCAACGC50350.049.6200524
CAACGCA54400.045.925915
AACGCAG55800.044.643876
TATCAAC58350.043.1892782
ACGCAGA61950.039.861237
CGCAGAG62950.039.112978
GTACATG45500.036.903071
TACATGG47350.034.258292
GTGGTAT13000.032.429971
GCAGAGT73700.032.42539
ACATGGG48950.031.2152883
TGGTATC14750.024.547972
GAGTACA37950.024.3250561
CATGGGG32350.023.056884
AGTACAT37400.023.0416182
CAGAGTA72150.020.9772039
TATGTCG1750.003684423620.6904325
Produced by FastQC (version 0.11.5)