FastQCFastQC Report
Thu 2 Jun 2016
DRR028576_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028576_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774637
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.048.8289571
TCAACGC17700.045.7659074
ATCAACG17950.045.1284943
CAACGCA20550.039.417685
GTATCAA22500.039.2459831
AACGCAG22750.036.2474066
TATCAAC25000.033.8618242
ACGCAGA23600.033.7021067
GTACATG33450.032.725591
CGCAGAG24550.032.1007238
TACATGG37700.027.2941862
CGACGAA2903.644618E-722.261553145
ACATGGG41700.022.2259353
GTATAGA11100.021.6961821
TCTGTCG5151.8189894E-1221.253318
GCAGAGT32850.020.8796189
TACGACC2104.128665E-420.8508764
TGCACCG2805.7589787E-620.8502795
CTGTCGC6250.019.8471899
GTATAGG7200.019.25811
Produced by FastQC (version 0.11.5)