FastQCFastQC Report
Thu 2 Jun 2016
DRR028576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774637
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19470.10971257783986246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18990.10700779934149914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23650.082.718471
GTATCAA42900.058.145781
ATCAACG45450.052.7469753
TCAACGC46050.051.902444
CAACGCA48950.048.8275225
AACGCAG50300.047.5170446
TATCAAC52650.045.9464042
ACGCAGA57800.041.100727
CGCAGAG57500.040.937288
GTGGTAT11850.032.5266151
GTACATG33000.031.7343711
GCAGAGT70050.031.6385639
GCCGTGC2802.6557245E-1028.453668
TGGTATC12550.028.2784632
TACATGG36950.026.4620062
ACATGGG40700.023.4899963
GAGTACA32350.023.1549761
TACGACC2455.1564475E-520.6935714
ACCGTAC1750.003681111420.693578
CAGAGTA65100.020.693579
Produced by FastQC (version 0.11.5)