FastQCFastQC Report
Thu 2 Jun 2016
DRR028575_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028575_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745258
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.051.449791
TCAACGC19700.046.297694
ATCAACG20100.045.3763433
GTACATG35900.039.429641
CAACGCA23200.038.9974865
GTATCAA24400.038.575841
AACGCAG25350.036.264596
TACATGG37900.034.4611172
TATCAAC27850.034.3211332
ACGCAGA25850.033.867697
CGCAGAG25950.033.1729628
ACATGGG42300.030.0140343
TATACCG1606.542489E-527.360346
CGTGTCG1906.8246827E-626.8756339
ATACCGC1658.069888E-526.5296927
CATGGGA19300.023.4395834
GCAGAGT34500.023.2588319
GTATATA15250.022.0091631
CATGGGG30200.020.7781584
GAGTACA32500.019.756721
Produced by FastQC (version 0.11.5)