FastQCFastQC Report
Thu 2 Jun 2016
DRR028575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745258
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20100.11516921853387865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.10668909697019008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.076.864831
GTATCAA40700.055.371531
TCAACGC44550.049.7490734
ATCAACG45200.049.1938933
CAACGCA47300.046.7035525
AACGCAG49500.044.6278426
TATCAAC53000.042.0906942
ACGCAGA55400.039.6135837
CGCAGAG56700.038.194378
GTACATG38750.036.7708441
TACATGG41300.032.2685172
GCAGAGT67350.031.186869
TGGTATC12950.029.0833782
ACATGGG46100.028.9086723
GTGGTAT14150.026.7156751
TATACCG2401.6129561E-624.1429316
ATACCGC2451.929866E-623.6502177
GAGTACA34700.022.4167981
CAGAGTA64950.021.9683959
AGTACAT34550.021.8020112
Produced by FastQC (version 0.11.5)