FastQCFastQC Report
Thu 2 Jun 2016
DRR028574_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028574_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121035
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG19700.043.3375473
TCAACGC19700.043.3375474
GGTATCA15750.042.1604771
GTATCAA21600.041.55241
CAACGCA23050.037.0381435
GTACATG40050.034.4353071
AACGCAG25000.034.149176
TATCAAC26500.032.492332
ACGCAGA25750.032.3028647
CGCAGAG25300.032.299858
GTACGGA1952.466495E-729.9357176
TACATGG45800.028.6781862
TCTGTCG7400.028.593638
CTGTCGC8650.025.3051059
ACATGGG53100.023.498833
TCTAGCG2404.1536565E-521.2829322
GTATAGG8750.020.8485871
GTATTAG22150.019.436721
ATAATAC16550.019.3998953
CTAGCGT2701.0276506E-418.9181653
Produced by FastQC (version 0.11.5)