FastQCFastQC Report
Thu 2 Jun 2016
DRR028574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121035
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.075.2924351
GTATCAA41550.058.9401631
TCAACGC44650.053.849544
ATCAACG45200.053.1942943
CAACGCA46300.051.9304925
AACGCAG50200.048.0403256
TATCAAC52400.046.2997782
CGCAGAG55800.042.6999248
ACGCAGA57550.041.527337
GTCGTAC1051.8291333E-434.6045461
GCAGAGT71450.032.840389
TAGATCG1000.0061083628.968465
GTACATG40850.027.9292981
GTACCGT1400.001006198725.8646956
GTATAGG7250.025.0584641
TGTACCG2901.2061719E-824.9728095
GTGGTAT14450.023.6364611
TACATGG47950.022.9572752
TGGTATC13100.022.6661592
TCGTACA2303.1706884E-522.0412182
Produced by FastQC (version 0.11.5)