FastQCFastQC Report
Thu 2 Jun 2016
DRR028573_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028573_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756576
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15150.052.5063631
ATCAACG22750.046.8348923
TCAACGC23050.045.5921024
GTATCAA25850.044.04151
CAACGCA26300.039.9557845
AACGCAG28250.037.1977736
GTACATG45400.037.13251
TACATGG47100.034.3977552
TATCAAC31550.034.2342152
ACGCAGA31150.033.2652367
CGCAGAG31150.032.3244448
CGACGAA3201.8189894E-1229.142048145
GTATTAG14300.028.5791991
ACATGGG52950.028.5300453
GAGTACA37550.023.127841
TATTAGG13600.022.537642
GCAGAGT41600.022.0991029
CATGGGG37350.021.6885454
AGTACAT37700.021.2936152
AATCCGC3859.762516E-920.8499436
Produced by FastQC (version 0.11.5)