FastQCFastQC Report
Thu 2 Jun 2016
DRR028573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1756576
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18090.10298444246078733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.10087807188530415No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.075.553941
GTATCAA44000.054.3807871
ATCAACG48450.048.4347763
TCAACGC49100.047.793584
CAACGCA51050.045.9679685
AACGCAG52050.045.0848166
TATCAAC56900.041.3692052
ACGCAGA58750.039.819957
CGCAGAG59550.039.041758
GTACATG41850.038.927351
GTGGTAT12400.035.7775341
TACATGG43700.034.8051832
GCAGAGT70700.031.5527619
ACATGGG47150.029.8006733
TGGTATC14450.029.5726492
GTATACG1409.815999E-425.9743231
CAGAGTA63250.023.8181959
TATATCG1600.00218841422.6337265
GAGTACA34800.022.1528151
GTATAGA10350.021.7832971
Produced by FastQC (version 0.11.5)