FastQCFastQC Report
Thu 2 Jun 2016
DRR028572_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028572_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332205
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22450.048.42424
ATCAACG23100.046.7457663
GGTATCA17050.041.5087741
GTATCAA27400.040.741161
CAACGCA27800.038.8427055
AACGCAG28750.037.8129846
ACGCAGA29100.036.8551337
CGCAGAG28850.036.6687248
TATCAAC30900.035.418112
GTACATG45600.029.9205321
TATACCG2853.0013325E-1028.1605015
GTACGGA2705.2077667E-927.0227036
TACATGG53950.025.5601222
GCAGAGT41700.023.2696159
GAGTACA43050.023.218811
GTATTAG23200.021.6996961
ATACCGT3457.0278475E-821.1482036
GTATATA16550.020.7201271
ACATGGG61000.020.5726953
ATACGCC3207.97947E-720.5203656
Produced by FastQC (version 0.11.5)