FastQCFastQC Report
Thu 2 Jun 2016
DRR028572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332205
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25850.066.405791
GTATCAA43000.057.6820221
ATCAACG47100.051.209333
TCAACGC47250.051.046764
CAACGCA50250.047.855055
AACGCAG52050.046.339286
TATCAAC56850.042.808952
ACGCAGA60400.039.4534267
CGCAGAG61500.038.7477538
GTACCGT1153.1810402E-431.4918336
GTACATG46200.030.2283041
GCAGAGT76650.030.049749
TACATGG52850.025.7655032
GTACGGA2502.300283E-623.1779886
ACCGACC2603.2337284E-622.2865288
ACATGGG58650.021.9825343
CAGAGTA72700.021.520149
CTAGCGT2404.3981432E-521.125773
CTAGTAC7000.020.6946323
TACCGTC1800.00433250220.1197817
Produced by FastQC (version 0.11.5)